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KMID : 1157520190150030113
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International Journal of Clinical Preventive Dentistry
2019 Volume.15 No. 3 p.113 ~ p.120
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Distribution of Oral Bacteria according to Sex and Age of Patients with Periodontal Disease
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Choi Ok-Sun
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Abstract
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The purpose of this study was to investigate the frequency of Porphyromonas gingivalis, Filifactor alocis, Campylobater rectus, Porphyromonas endodontalis, Tenerella forsythensis, and Actinobacillus actinomycetemcomitans, which are causative bacteria of periodontal disease, in order to investigate the distribution pattern of periodontal disease bacteria from children aged less than 10 years to old people more than 61 years. For this purpose, plaques were collected from the dentate gyrus and the 16s rRNA gene was amplified by polymerase chain reaction (PCR) to investigate the distribution of periodontal pathogens by sex and age. The results obtained in this study are the basic data for improving oral health and quality of life. Among the various kinds of bacteria, Hemophilus aphrophilus, P. gingivalis, A. actinomycetemcomitans, T. forsythensis, Treponema denticola, Prevotella intermedia, Micromonas micros, Fusobacterium nucleatum, C. rectus, and Eikenella corrodens are recognized as a main causative organisms of periodontal disease. PCR is more specific and sensitive than culture method, and it is a good method to detect DNA directly from oral gingival epithelium and oral plaque. At least the population of bacteria can be diagnosed.
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KEYWORD
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oral bacteria, periodontitis, preventive care, subgingival plaque, polymerase chain reaction
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